Light-dependent de-activation/re-activation of Anabaena variabilis ferredoxin: NADP+ reductase
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Light-dependent de-activation/re-activation of Anabaena variabilis ferredoxin: NADP+ reductase.
The activity of ferredoxin: NADP+ reductase (FNR) was found to decline to approximately 20% maximal levels with little or no loss in enzyme levels when cultures of the cyanobacterium Anabaena variabilis were maintained in the stationary phase of growth. Re-activation of enzyme activity occurred when cells were diluted into either fresh or re-utilized media and illuminated. This reversible de-ac...
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The expression of ferredoxin-NADP+ reductase (FNR) from Anabaena sp. PCC 7119 in heterocysts and vegetative cells has been quantified. Specific reductase activity in heterocysts was approximately 10 times higher than in vegetative cells, corresponding to the increased FNR protein content. This was confirmed by immunoquantification of the FNR protein from whole filaments of Anabaena sp. PCC 7120...
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Ferredoxin-NADP+ reductase (FNR) catalyzes the electron transfer from ferredoxin to NADP+ via its flavin FAD cofactor. To get further insights in the architecture of the transient complexes produced during the hydride transfer event between the enzyme and the NADP+ coenzyme we have applied NMR spectroscopy using Saturation Transfer Difference (STD) techniques to analyze the interaction between ...
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1 7 W . K R E U T Z U. W . MENKE, Z . N a t u r f o r s c h . 1 7 b , 6 7 5 [1962], 18 W. KREUTZ, Z. Naturforsch. 18 b, 1098 [1963] ; 19 b, 441 [1964], 19 W. MENKE, in: Photosynthesis Mechanisms in green Plants, Publ. 1145, Nat. Acad. Sci.-Nat. Res. Council, Publ. 1963, p. 537. 20 W. MENKE, in: Biochemistry of Chloroplasts, Vol. I, (ed. T. GOODWIN), p. 3, Acad. Press, London and New York 1967. ...
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Steady state rates of NADP reduction decline upon commencement of nitrite reduction in reconstituted chloroplast preparations. Similarly, steady state rates of nitrite reduction are lower, but not zero, during concurrent NADP reduction. These results imply that competition for substrate occurs and suggest that nitrite reduction can successfully compete for reduced ferredoxin, even at high rates...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1991
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2740781